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Prion Protein (PrP) (143-153) antibody, Internal

Anti-Prion Protein (PrP) (143-153) antibody, Internal used in Western Blot (WB). GTX89339

Cat. No. GTX89339

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all Prion Protein (PrP) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89339-PEP).

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SDYEDRYYREN, from the internal region of the protein sequence according to NP_000302.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prion protein , ASCR , AltPrP , CD230 , CJD , GSS , KURU , PRIP , PrP , PrP27-30 , PrP33-35C , PrPc , p27-30

Cellular Localization

Golgi apparatus , Mitochondrion outer membrane

Background

The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-Prion Protein (PrP) (143-153) antibody, Internal used in Western Blot (WB). GTX89339

GTX89339 WB Image

WB analysis of human brain lysate using GTX89339 Prion Protein (PrP) (143-153) antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Nurul Sulimai et al. Int J Mol Sci 2021; 22 (5) Fibrinogen Interaction with Astrocyte ICAM-1 and PrPC Results in the Generation of ROS and Neuronal Death.
Application : WB
Nurul Sulimai et al. Biomolecules 2021; 11 (9) The Effects of Fibrinogen's Interactions with Its Neuronal Receptors, Intercellular Adhesion Molecule-1 and Cellular Prion Protein.
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399