APPLICATION
Application Note
ELISA: use at a concentration of 4 ng/ml. Western blot (non-reducing conditions): use at a concentration of 10 μg/ml. Optimal dilutions / concentrations should be determined by the end user.
Product Note
This antibody detects:Human recombinant prion protein.Bovine recombinant, native and PrPres proteinase K treated prion protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Bovine
Immunogen
Recombinant bovine prion protein. Immunization was performed according to the protocol described by Hofmann, J. et al.
Purification
Immunogen affinity purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
prion protein , ASCR , AltPrP , CD230 , CJD , GSS , KURU , PRIP , PrP , PrP27-30 , PrP33-35C , PrPc , p27-30
Cellular Localization
Golgi apparatus , Mitochondrion outer membrane
Background
The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
![Anti-Prion Protein (PrP) antibody [1E5 / G6] used in Western Blot (WB). GTX22879](/templates/default/js/lightbox/images/loading.gif "Anti-Prion Protein (PrP) antibody [1E5 / G6] used in Western Blot (WB). GTX22879")
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GTX22879 WB Image
GTX22879 at a 1mg/ml concentration staining~23 kDa (mature) and ~28 kDa (pro-peptide)native bovine prion proteinfrom brain (M. oblongata; 100 mg comprising 10% homogenised brain,diluted 1:10 in buffer, 10ml loaded) by Western blot (non-reducing conditions
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REFERENCE
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REVIEW
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![Anti-Prion Protein (PrP) antibody [1E5 / G6] used in Western Blot (WB). GTX22879](/upload/website/prouct_img/small/GTX22879/Prion-protein-PrP-antibody-1E5-G6-GTX22879-WB-1_w_23060620_194.webp "Anti-Prion Protein (PrP) antibody [1E5 / G6] used in Western Blot (WB). GTX22879")
![Anti-Prion Protein (PrP) antibody [1E5 / G6] used in Western Blot (WB). GTX22879](/upload/website/prouct_img/normal/GTX22879/Prion-protein-PrP-antibody-1E5-G6-GTX22879-WB-1_w_23060620_194.webp "Anti-Prion Protein (PrP) antibody [1E5 / G6] used in Western Blot (WB). GTX22879")