GeneTex
United States (US)

Prion Protein (PrP) antibody

GTX101063_40254_20141009_IHC_M_19011506_324.jpg
GTX101063_WB_19011506_877.jpg
GTX101063_40254_WB_M_19011506_225.jpg

Cat No. GTX101063

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

IMR32 , Neuro2A , NIH-3T3 , C2C12

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ASCR antibody, CD230 antibody, CJD antibody, GSS antibody, MGC26679 antibody, PRIP antibody, PrP antibody, PrP27-30 antibody, PrP33-35C antibody, PrPc antibody, prion antibody, PRNP antibody, prion protein PrP antibody, prion-related protein antibody, CD230 antigen antibody, prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia) antibody, major prion protein antibody, p27-30 antibody, prion protein antibody

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor , Golgi apparatus

Background

The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101063_40254_20141009_IHC_M_19011506_324.jpg

GTX101063 IHC-P Image

PRP antibody detects PRP protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PRP antibody (GTX101063) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101063_WB_19011506_877.jpg

GTX101063 WB Image

Sample (30 ug of whole cell lysate)
A:IMR32
12% SDS PAGE
GTX101063 diluted at 1:1000

GTX101063_40254_WB_M_19011506_225.jpg

GTX101063 WB Image

PRP antibody detects PRNP protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
PRP antibody (GTX101063) dilution: 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169