APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Neuro2A , NIH-3T3 , C2C12 , IMR32
Predict Reactivity
Rat, Rabbit, Sheep, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.36 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Prion Protein (PrP). The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10617796
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
prion protein , ASCR , AltPrP , CD230 , CJD , GSS , KURU , PRIP , PrP , PrP27-30 , PrP33-35C , PrPc , p27-30
Cellular Localization
Cell membrane; Lipid-anchor , GPI-anchor , Golgi apparatus
Background
The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101063 WB Image
Sample (30 ug of whole cell lysate) A:IMR32 12% SDS PAGE GTX101063 diluted at 1:1000
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GTX101063 WB Image
PRP antibody detects PRNP protein by Western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract C. 30 μg C2C12 whole cell lysate/extract 12 % SDS-PAGE PRP antibody (GTX101063) dilution: 1:1000
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GTX101063 IHC-P Image
PRP antibody detects PRP protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. PRP antibody (GTX101063) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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REVIEW
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