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Proenkephalin antibody, Internal

Anti-Proenkephalin antibody, Internal used in IHC (Frozen sections) (IHC-Fr). GTX88917
Anti-Proenkephalin antibody, Internal used in Western Blot (WB). GTX88917

Cat. No. GTX88917

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human
Package
100 μg ($399)
See all proenkephalin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-Fr 0.3-1μg/ml

Note :

IHC-Fr
Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus.
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88917-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RSHHQDGSDNEE, from the internal region of the protein sequence according to NP_006202.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proenkephalin , PE , PENK-A

Cellular Localization

Secreted

Background

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES

Anti-Proenkephalin antibody, Internal used in IHC (Frozen sections) (IHC-Fr). GTX88917

GTX88917 IHC-Fr Image

IHC-Fr analysis of PFA-perfused human hypothalamus using GTX88917 Proenkephalin antibody, Internal.
Antigen retrieval : citrate buffer pH 6 at 80C for 30min
Dilution : 0.3μg/ml

Anti-Proenkephalin antibody, Internal used in Western Blot (WB). GTX88917

GTX88917 WB Image

WB analysis of human adrenal gland lysate using GTX88917 Proenkephalin antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399