Profilin 1 antibody [1D5]

Cat. No. GTX83903

Host	Mouse
Clonality	Monoclonal
Clone Name	1D5
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat, Dog, Monkey
Package	100 μl ($519)

See all Profilin 1 products

Datasheet
Document

View product citations for antibody GTX83903 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:200-1:2000
ICC/IF	1:100
IHC-P	1:50
FACS	1:100

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

profilin 1 , ALS18

Cellular Localization

Cytoplasm, cytoskeleton

Background

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

Database

Gene ID: 5216 PFN1
UniProt: P07737 PFN1

Research Area

DATA IMAGES

GTX83903 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83903 Profilin 1 antibody [1D5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83903 WB Image

WB analysis of various cell lines using GTX83903 Profilin 1 antibody [1D5].
Loading : 35 ug per lane

GTX83903 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Profilin 1 plasmid using GTX83903 Profilin 1 antibody [1D5].

GTX83903 FACS Image

FACS analysis of Jurkat cells using GTX83903 Profilin 1 antibody [1D5].
Red : Primary antibody
Blue : Negative control antibody

GTX83903 WB Image

WB analysis of HEK293T cells transfected with Profilin 1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83903 Profilin 1 antibody [1D5].
Loading : 5 ug per lane

GTX83903 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83903 Profilin 1 antibody [1D5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83903 FACS Image

FACS analysis of HEK293T cells transfected with either Profilin 1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83903 Profilin 1 antibody [1D5].

GTX83903 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83903 Profilin 1 antibody [1D5].
Loading : 10 ug per lane
Dilution : 1:200

GTX83903 WB Image

WB analysis of various samples using GTX83903 Profilin 1 antibody [1D5].
Loading : 10 ug per lane
Dilution : 1:200

GTX83903 FACS Image

FACS analysis of HeLa cells using GTX83903 Profilin 1 antibody [1D5].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

Profilin 1 antibody [1D5]

Cat. No. GTX83903

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83903 IHC-P Image

GTX83903 WB Image

GTX83903 ICC/IF Image

GTX83903 FACS Image

GTX83903 WB Image

GTX83903 IHC-P Image

GTX83903 FACS Image

GTX83903 WB Image

GTX83903 WB Image

GTX83903 FACS Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.