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Profilin 1 antibody [2D2]

Anti-Profilin 1 antibody [2D2] used in Flow cytometry (FACS). GTX83902
Anti-Profilin 1 antibody [2D2] used in Western Blot (WB). GTX83902
Anti-Profilin 1 antibody [2D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83902
Anti-Profilin 1 antibody [2D2] used in Western Blot (WB). GTX83902
Anti-Profilin 1 antibody [2D2] used in IHC (Paraffin sections) (IHC-P). GTX83902
Anti-Profilin 1 antibody [2D2] used in Flow cytometry (FACS). GTX83902
Anti-Profilin 1 antibody [2D2] used in IHC (Paraffin sections) (IHC-P). GTX83902
Anti-Profilin 1 antibody [2D2] used in IHC (Paraffin sections) (IHC-P). GTX83902
Anti-Profilin 1 antibody [2D2] used in Flow cytometry (FACS). GTX83902

Cat No. GTX83902

Host

Mouse

Clonality

Monoclonal

Clone Name

2D2

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Rat, Dog, Monkey
Package
100 μl ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

profilin 1 , ALS18

Cellular Localization

Cytoplasm, cytoskeleton

Background

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

Database

Research Area

DATA IMAGES

Anti-Profilin 1 antibody [2D2] used in Flow cytometry (FACS). GTX83902

GTX83902 FACS Image

FACS analysis of HEK293T cells transfected with either Profilin 1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83902 Profilin 1 antibody [2D2].

Anti-Profilin 1 antibody [2D2] used in Western Blot (WB). GTX83902

GTX83902 WB Image

WB analysis of HEK293T cells transfected with Profilin 1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83902 Profilin 1 antibody [2D2].
Loading : 5 ug per lane

Anti-Profilin 1 antibody [2D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83902

GTX83902 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Profilin 1 plasmid using GTX83902 Profilin 1 antibody [2D2].

Anti-Profilin 1 antibody [2D2] used in Western Blot (WB). GTX83902

GTX83902 WB Image

WB analysis of various cell lines using GTX83902 Profilin 1 antibody [2D2].
Loading : 35 ug per lane

Anti-Profilin 1 antibody [2D2] used in IHC (Paraffin sections) (IHC-P). GTX83902

GTX83902 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83902 Profilin 1 antibody [2D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Profilin 1 antibody [2D2] used in Flow cytometry (FACS). GTX83902

GTX83902 FACS Image

FACS analysis of Jurkat cells using GTX83902 Profilin 1 antibody [2D2].
Red : Primary antibody
Blue : Negative control antibody

Anti-Profilin 1 antibody [2D2] used in IHC (Paraffin sections) (IHC-P). GTX83902

GTX83902 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83902 Profilin 1 antibody [2D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Profilin 1 antibody [2D2] used in IHC (Paraffin sections) (IHC-P). GTX83902

GTX83902 IHC-P Image

IHC-P analysis of human prostate tissue using GTX83902 Profilin 1 antibody [2D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-Profilin 1 antibody [2D2] used in Flow cytometry (FACS). GTX83902

GTX83902 FACS Image

FACS analysis of HeLa cells using GTX83902 Profilin 1 antibody [2D2].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

There are currently no references for Profilin 1 antibody [2D2] (GTX83902). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Profilin 1 antibody [2D2] (GTX83902). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419