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Profilin 1 antibody [N1C3]

Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072
Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072
Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072
Anti-Profilin 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102072
Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072

Cat. No. GTX102072

Reference 5
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Profilin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse spleen , Rat spleen

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Profilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241179

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

profilin 1 , ALS18

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072

GTX102072 WB Image

Profilin 1 antibody [N1C3] detects Profilin 1 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
D. 30 μg THP-1 whole cell lysate/extract
E. 30 μg HL-60 whole cell lysate/extract
F. 30 μg NCI-H929 whole cell lysate/extract
15 % SDS-PAGE
Profilin 1 antibody [N1C3] (GTX102072) dilution: 1:10000

Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072

GTX102072 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
15% SDS PAGE
GTX102072 diluted at 1:1000

Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072

GTX102072 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 1 antibody [N1C3] (GTX102072) diluted at 1:500.

Anti-Profilin 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102072

GTX102072 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-231 xenograft, using Profilin 1(GTX102072) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072

GTX102072 WB Image

Profilin 1 antibody detects Profilin 1 protein by Western blot analysis.
A. 50 μg mouse spleen lysate/extract
15 % SDS-PAGE
Profilin 1 antibody (GTX102072) dilution: 1:500

REFERENCE

Application Reference

Ayoubi R et al. F1000Res 2023; 12 : 348 The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence.
Shirakawa J et al. JBMR Plus 2019; 3 (6) : e10130 Profilin 1 Negatively Regulates Osteoclast Migration in Postnatal Skeletal Growth
Application : ICC/IF
Reactivity : Mouse
Chang SJ et al. Arch Biochem Biophys 2018; 647 : 10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Sun SP et al. Nano Futures 2017; (Epub) A Co-Delivery Nanosystem of Chemotherapeutics and DNAzyme Overcomes Cancer Drug Resistance and Metastasis.
Application : WB
Reactivity : Human
Wu CL et al. Electrophoresis 2013; 34 (3) : 388-96 Hyaluronic acid-dependent protection against alkali-burned human corneal cells.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

Profilin 1 antibody [N1C3] Cat. No. GTX102072

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072
Date : Anonymous submitted on 09-Nov-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and PFN1 KO cells
Amount used : 50 μg
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169