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Profilin 1 antibody [N1C3]

Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072
Anti-Profilin 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102072
Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072
Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072
Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072

Cat. No. GTX102072

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse spleen , Rat spleen

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Profilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241179

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

profilin 1 , ALS18

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072

GTX102072 WB Image

Profilin 1 antibody [N1C3] detects Profilin 1 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
D. 30 μg THP-1 whole cell lysate/extract
E. 30 μg HL-60 whole cell lysate/extract
F. 30 μg NCI-H929 whole cell lysate/extract
15 % SDS-PAGE
Profilin 1 antibody [N1C3] (GTX102072) dilution: 1:10000

Anti-Profilin 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX102072

GTX102072 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-231 xenograft, using Profilin 1(GTX102072) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072

GTX102072 WB Image

Profilin 1 antibody detects Profilin 1 protein by Western blot analysis.
A. 50 μg mouse spleen lysate/extract
15 % SDS-PAGE
Profilin 1 antibody (GTX102072) dilution: 1:500

Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072

GTX102072 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
15% SDS PAGE
GTX102072 diluted at 1:1000

Anti-Profilin 1 antibody [N1C3] used in Western Blot (WB). GTX102072

GTX102072 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 1 antibody [N1C3] (GTX102072) diluted at 1:500.

REFERENCE

REVIEW

Profilin 1 antibody [N1C3] Cat. No. GTX102072

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 09-Nov-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and PFN1 KO cells
Amount used : 50 μg
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169