GeneTex
United States (US)

Profilin 1 antibody [N1C3]

GTX102072_40660_WB_19011506_232.jpg
GTX102072_40660_20160512_WB_R_spleen_19011506_974.jpg
GTX102072_40660_IHC_19011506_739.jpg
GTX102072_40660_WB_M_spleen_19011506_582.jpg

Cat No. GTX102072

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse spleen , Rat spleen

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Profilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Profilin 1,Als18,Pfn1

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102072_40660_WB_19011506_232.jpg

GTX102072 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
15% SDS PAGE
GTX102072 diluted at 1:1000

GTX102072_40660_20160512_WB_R_spleen_19011506_974.jpg

GTX102072 WB Image

Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 1 antibody [N1C3] (GTX102072) diluted at 1:500.

GTX102072_40660_IHC_19011506_739.jpg

GTX102072 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-231 xenograft, using Profilin 1(GTX102072) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102072_40660_WB_M_spleen_19011506_582.jpg

GTX102072 WB Image

Profilin 1 antibody detects Profilin 1 protein by Western blot analysis.
A. 50 µg mouse spleen lysate/extract
15 % SDS-PAGE
Profilin 1 antibody (GTX102072) dilution: 1:500

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf