APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , H1299 , mouse brain , rat brain
Product Note
This antibody may cross-react with human Profilin 1.
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Profilin 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951194
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
profilin 2 , D3S1319E , PFL
Cellular Localization
Cytoplasm , cytoskeleton
Background
The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX108589 WB Image
Sample(30 ug whole cell lysate) A: A431(GTX27909) B: H1299 12% SDS PAGE GTX108589 diluted at 1:500
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GTX108589 WB Image
Sample (50 ug of whole cell lysate) A: mouse brain 15% SDS PAGE GTX108589 diluted at 1:1000
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GTX108589 IHC-P Image
Profilin 2 antibody detects PFN2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Profilin 2 antibody (GTX108589) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX108589 WB Image
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 2 antibody (GTX108589) diluted at 1:1000.
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REFERENCE
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REVIEW
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