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Profilin 2 antibody

Anti-Profilin 2 antibody used in Western Blot (WB). GTX108589
Anti-Profilin 2 antibody used in Western Blot (WB). GTX108589
Anti-Profilin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX108589
Anti-Profilin 2 antibody used in Western Blot (WB). GTX108589

Cat No. GTX108589

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

A431 , H1299 , mouse brain , rat brain

Product Note

This antibody may cross-react with human Profilin 1.

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Profilin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951194

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

profilin 2 , D3S1319E , PFL

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Profilin 2 antibody used in Western Blot (WB). GTX108589

GTX108589 WB Image

Sample(30 ug whole cell lysate)
A: A431(GTX27909)
B: H1299
12% SDS PAGE
GTX108589 diluted at 1:500

Anti-Profilin 2 antibody used in Western Blot (WB). GTX108589

GTX108589 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX108589 diluted at 1:1000

Anti-Profilin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX108589

GTX108589 IHC-P Image

Profilin 2 antibody detects PFN2 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
Profilin 2 antibody (GTX108589) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Profilin 2 antibody used in Western Blot (WB). GTX108589

GTX108589 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Profilin 2 antibody (GTX108589) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169