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Progesterone Receptor antibody [N1], N-term

Orthogonal
Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051
Anti-Progesterone Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116051
Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051

Cat. No. GTX116051

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Progesterone Receptor products
PRODUCT

Summary

The progesterone receptor (PR) is a well-known estrogen receptor (ER) target gene that is expressed in over two-thirds of ER-positive breast cancers. PR protein has a predicted molecular weight of 99kDa. It acts as a transcriptional regulator and activator of signal transduction pathways (i.e., MAPK, PI3K/Akt, and c-Src) that can drive proliferative signaling in breast cancer cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Progesterone Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037757

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

progesterone receptor , NR3C3 , PR

Cellular Localization

Nucleus , Cytoplasm , Isoform A: Nucleus

Background

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform A has not been clearly determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051

GTX116051 IHC-P Image

Orthogonal

Immunohistochemical analysis of paraffin-embedded human breast cancer using Progesterone Receptor antibody (GTX116051) diluted at 1:500.
Representative data showing negative staining in PR-negative breast cancer (left) and nuclear staining in PR-positive breast cancer (right).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Progesterone Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116051

GTX116051 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using Progesterone receptor(GTX116051) antibody at 1:200 dilution.

Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051

GTX116051 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Progesterone receptor(GTX116051) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Yin et al. Biomedical Research 2017; (Epub) Altered expression of sex hormone receptors in keratoconus corneas.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169