PRODUCT
Summary
The progesterone receptor (PR) is a well-known estrogen receptor (ER) target gene that is expressed in over two-thirds of ER-positive breast cancers. PR protein has a predicted molecular weight of 99kDa. It acts as a transcriptional regulator and activator of signal transduction pathways (i.e., MAPK, PI3K/Akt, and c-Src) that can drive proliferative signaling in breast cancer cells.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.33 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Progesterone Receptor. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037757
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
progesterone receptor , NR3C3 , PR
Cellular Localization
Nucleus , Cytoplasm , Isoform A: Nucleus
Background
This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. The location of transcription initiation for isoform A has not been clearly determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051](/templates/default/js/lightbox/images/loading.gif "Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051")
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GTX116051 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human breast cancer using Progesterone Receptor antibody (GTX116051) diluted at 1:500.
Representative data showing negative staining in PR-negative breast cancer (left) and nuclear staining in PR-positive breast cancer (right).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX116051 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Progesterone receptor(GTX116051) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX116051 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using Progesterone receptor(GTX116051) antibody at 1:200 dilution.
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REFERENCE
REVIEW
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![Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051](/upload/website/prouct_img/small/GTX116051/GTX116051_40135_20160307_IHC-P_18121410_737.jpg "Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051")
![Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051](/upload/website/prouct_img/small/GTX116051/GTX116051_IHC_18121410_616.jpg "Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051")
![Anti-Progesterone Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116051](/upload/website/prouct_img/small/GTX116051/GTX116051_IFA_18121410_638.jpg "Anti-Progesterone Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116051")
![Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051](/upload/website/prouct_img/normal/GTX116051/GTX116051_40135_20160307_IHC-P_18121410_737.jpg "Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051")
![Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051](/upload/website/prouct_img/normal/GTX116051/GTX116051_IHC_18121410_616.jpg "Anti-Progesterone Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX116051")
![Anti-Progesterone Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116051](/upload/website/prouct_img/normal/GTX116051/GTX116051_IFA_18121410_638.jpg "Anti-Progesterone Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116051")