Progesterone Receptor (phospho Ser190) antibody [1154]

Cat. No. GTX30164

Host	Mouse
Clonality	Monoclonal
Clone Name	1154
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IP, ELISA
Reactivity	Human, Mouse
Package	100 μg ($359)

See all Progesterone Receptor products

Datasheet
Document

View product citations for antibody GTX30164 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	4 μg/ml
ICC/IF	1:10-1:100
IHC-P	2-4 μg/ml
IP	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

99 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

buffer, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

progesterone receptor , NR3C3 , PR

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Sep 2015]

Database

Gene ID: 5241 PGR
UniProt: P06401 PGR

Research Area

DATA IMAGES

GTX30164 ICC/IF Image

ICC/IF analysis of untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel) using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute

GTX30164 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX30164 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX30164 IHC-P Image

IHC-P analysis of human uterus tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX30164 ICC/IF Image

ICC/IF analysis of T47D cells using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

GTX30164 WB Image

WB analysis of 20 ug of nuclear (N) or cytoplasmic (C) of T47D cell lysate untreated (-) or stimulated (+) with 100 nM promegestone (R5020) for 1 hour using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154] at 4 μg/ml (upper panel) or a Progesterone Receptor mouse monoclonal antibody (lower pane) . This antibody detects both phosphorylated isoform A and isoform B.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 359

Qty

Progesterone Receptor (phospho Ser190) antibody [1154]

Cat. No. GTX30164

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX30164 ICC/IF Image

GTX30164 IHC-P Image

GTX30164 IHC-P Image

GTX30164 IHC-P Image

GTX30164 ICC/IF Image

GTX30164 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.