GeneTex
United States (US)

Progesterone Receptor (phospho Ser190) antibody [1154]

Orthogonal
GTX30164_1329_IHC-P_19011519_815.jpg
GTX30164_1330_IHC-P_19011519_767.jpg
GTX30164_1331_IHC-P_19011519_165.jpg
GTX30164_722_ICC-IF_19011519_581.jpg
GTX30164_723_ICC-IF_19011519_942.jpg
GTX30164_1842_WB_19011519_280.jpg

Cat No. GTX30164

Host Mouse
Clonality Monoclonal
Clone Name 1154
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 4 μg/ml
ICC/IF 1:10-1:100
IHC-P 2-4 μg/ml
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

99 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Buffer, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Progesterone Receptor,Nr3C3,Pr,Pgr

Cellular Localization

Cytoplasm,Mitochondrion outer membrane,Nucleus

Background

This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES
GTX30164_1329_IHC-P_19011519_815.jpg

GTX30164 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX30164_1330_IHC-P_19011519_767.jpg

GTX30164 IHC-P Image

IHC-P analysis of human uterus tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX30164_1331_IHC-P_19011519_165.jpg

GTX30164 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX30164_722_ICC-IF_19011519_581.jpg

GTX30164 ICC/IF Image

ICC/IF analysis of T47D cells using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

GTX30164_723_ICC-IF_19011519_942.jpg

GTX30164 ICC/IF Image

Orthogonal

ICC/IF analysis of untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel) using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute

GTX30164_1842_WB_19011519_280.jpg

GTX30164 WB Image

Orthogonal

WB analysis of 20 ug of nuclear (N) or cytoplasmic (C) of T47D cell lysate untreated (-) or stimulated (+) with 100 nM promegestone (R5020) for 1 hour using GTX30164 Progesterone Receptor (phospho Ser190) antibody [1154] at 4 µg/ml (upper panel) or a Progesterone Receptor mouse monoclonal antibody (lower pane) . This antibody detects both phosphorylated isoform A and isoform B.

Package List Price ($)
$ 319