GeneTex
United States (US)

Progesterone Receptor (phospho Ser294) antibody [608]

Progesterone-Receptor-phospho-Ser294-antibody-608__GTX30165-1_18121411_688.jpg

Cat No. GTX30165

Host Mouse
Clonality Monoclonal
Clone Name 608
Isotype IgG1
Application WB, IHC
Reactivity Human
APPLICATION

Calculated MW

90/120 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser294 conjugated to KLH

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PGR, 607311, P06401, PR, NR3C3, 5241, Progesterone Receptor (phospho S294), Progesterone Receptor (phospho Ser294), Progesterone Receptor (pS294), Progesterone Receptor (pSer294), Progesterone Receptor phospho S294, Progesterone Receptor phospho Ser294, Phospho Progesterone Receptor (S294), Phospho Progesterone Receptor (Ser294), Phospho Progesterone Receptor (pS294), Phospho Progesterone Receptor (pSer294)

Cellular Localization

Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases. Mainly nuclear.

Background

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120kDa protein (Form B) or a 94kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. When bound to DNA, PgR can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.

Database

Research Area

DATA IMAGES
Progesterone-Receptor-phospho-Ser294-antibody-608__GTX30165-1_18121411_688.jpg

GTX30165 WB Image

Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser294 using Progesterone Receptor (phospho Ser294) antibody [608] (GTX30165). The immunolabeling is blocked by the phosphopeptide used as the antigen (not shown).

Package List Price ($)
$ 329