Prohibitin 2 antibody

Name: Prohibitin 2 antibody
Brand: GeneTex
SKU: GTX102100
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-Prohibitin 2 antibody used in Western Blot (WB). GTX102100

Anti-Prohibitin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX102100

Anti-Prohibitin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102100

Cat. No. GTX102100

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Prohibitin 2 products

View product citations for antibody GTX102100 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Mouse brain , H1299 , HeLa

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Prohibitin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037852

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prohibitin 2 , BAP , BCAP37 , Bap37 , PNAS-141 , REA , hBAP , p22

Cellular Localization

Mitochondrion inner membrane , Cytoplasm , Nucleus

Background

Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.

Database

Gene ID: 11331 PHB2
UniProt: Q99623 PHB2

Research Area

DATA IMAGES

GTX102100 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
B: HeLa
10% SDS PAGE
GTX102100 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102100 IHC-P Image

Prohibitin 2 antibody detects Prohibitin 2 protein at cytoplasm and nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder .
Prohibitin 2 antibody (GTX102100) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102100 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX102100 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102100 IHC-P Image

Prohibitin 2 antibody detects PHB2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Prohibitin 2 antibody (GTX102100) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102100 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using REA (GTX102100) antibody at 1:200 dilution.

REFERENCE

Application Reference

Heng-Hsiung Wu et al. Journal of Food and Drug Analysis 2019; (Epub) Involvement of prohibitin 1 and prohibitin 2 upregulation in cBSA-induced podocyte cytotoxicity.

Application : IHC-P

Reactivity : Mouse

Liu YH et al. Phytomedicine 2018; 46 : 184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.

Application : ICC/IF

Reactivity : Human

Martinez E et al. Int J Biochem Cell Biol 2015; 59 : 62-72 Methyl donor deficiency in H9c2 cardiomyoblasts induces ER stress as an important part of the proteome response.

Application : WB

Reactivity : Rat

Martinez E et al. J Nutr Biochem 2013; 24 (7) : 1241-50 Myocardium proteome remodelling after nutritional deprivation of methyl donors.

Application : WB

Reactivity : Rat

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