GeneTex
United States (US)

Prohibitin 2 antibody

GTX102100_40135_WB_M_brain_19011506_843.jpg
GTX102100_40135_IHC_19011506_142.jpg
GTX102100_WB_19011506_776.jpg
GTX102100_40135_20150313_IHC_M_19011506_196.jpg
GTX102100_IFA_19011506_447.jpg

Cat No. GTX102100

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

H1299 , HeLa , mouse brain

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Prohibitin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BAP antibody, BCAP37 antibody, Bap37 antibody, MGC117268 antibody, PNAS-141 antibody, REA antibody, p22 antibody, PHB2 antibody, B-cell receptor-associated protein BAP37 antibody, prohibitin-2 antibody, B-cell associated protein antibody, D-prohibitin antibody, repressor of estrogen receptor activity antibody, prohibitin 2 antibody

Cellular Localization

Mitochondrion inner membrane (By similarity) , Cytoplasm (By similarity) , Nucleus (By similarity)

Background

Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.

Database

Research Area

DATA IMAGES
GTX102100_40135_WB_M_brain_19011506_843.jpg

GTX102100 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX102100 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102100_40135_IHC_19011506_142.jpg

GTX102100 IHC-P Image

Prohibitin 2 antibody detects PHB2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Prohibitin 2 antibody (GTX102100) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102100_WB_19011506_776.jpg

GTX102100 WB Image

Sample (30 µg of whole cell lysate)
A: H1299
B: HeLa
10% SDS PAGE
GTX102100 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102100_40135_20150313_IHC_M_19011506_196.jpg

GTX102100 IHC-P Image

Prohibitin 2 antibody detects Prohibitin 2 protein at cytoplasm and nucleus on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder .
Prohibitin 2 antibody (GTX102100) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102100_IFA_19011506_447.jpg

GTX102100 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using REA (GTX102100) antibody at 1:200 dilution.

REFERENCE
REVIEW

Prohibitin 2 antibody Cat No. GTX102100

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 22-Sep-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : human embryonic stem cells
Amount used : 30μg
Blocking : 5% BSA, 18°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169