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Prohibitin antibody [5H7]

Anti-Prohibitin antibody [5H7] used in IHC (Paraffin sections) (IHC-P). GTX60495
Anti-Prohibitin antibody [5H7] used in Flow cytometry (FACS). GTX60495
Anti-Prohibitin antibody [5H7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60495
Anti-Prohibitin antibody [5H7] used in IHC (Paraffin sections) (IHC-P). GTX60495
Anti-Prohibitin antibody [5H7] used in ELISA (ELISA). GTX60495
Anti-Prohibitin antibody [5H7] used in Western Blot (WB). GTX60495
Anti-Prohibitin antibody [5H7] used in IHC (Paraffin sections) (IHC-P). GTX60495
Anti-Prohibitin antibody [5H7] used in IHC (Paraffin sections) (IHC-P). GTX60495
Anti-Prohibitin antibody [5H7] used in Western Blot (WB). GTX60495

Cat No. GTX60495

Host

Mouse

Clonality

Monoclonal

Clone Name

5H7

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PHB expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887975

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

prohibitin , HEL-215 , HEL-S-54e , PHB1

Cellular Localization

Mitochondrion inner membrane

Background

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-Prohibitin antibody [5H7] used in IHC (Paraffin sections) (IHC-P). GTX60495

GTX60495 IHC-P Image

IHC-P analysis of rectum cancer tissue using GTX60495 Prohibitin antibody [5H7].

Anti-Prohibitin antibody [5H7] used in Flow cytometry (FACS). GTX60495

GTX60495 FACS Image

FACS analysis of MCF-7 cells using GTX60495 Prohibitin antibody [5H7].
Blue : Prohibitin
Red : negative control

Anti-Prohibitin antibody [5H7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60495

GTX60495 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX60495 Prohibitin antibody [5H7].
Green : Prohibitin
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Prohibitin antibody [5H7] used in IHC (Paraffin sections) (IHC-P). GTX60495

GTX60495 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60495 Prohibitin antibody [5H7].

Anti-Prohibitin antibody [5H7] used in ELISA (ELISA). GTX60495

GTX60495 ELISA Image

ELISA analysis of antigen using GTX60495 Prohibitin antibody [5H7].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-Prohibitin antibody [5H7] used in Western Blot (WB). GTX60495

GTX60495 WB Image

WB analysis of human Prohibitin (AA: 68-259) recombinant protein using GTX60495 Prohibitin antibody [5H7].

Anti-Prohibitin antibody [5H7] used in IHC (Paraffin sections) (IHC-P). GTX60495

GTX60495 IHC-P Image

IHC-P analysis of stomach cancer tissue using GTX60495 Prohibitin antibody [5H7].

Anti-Prohibitin antibody [5H7] used in IHC (Paraffin sections) (IHC-P). GTX60495

GTX60495 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60495 Prohibitin antibody [5H7].

Anti-Prohibitin antibody [5H7] used in Western Blot (WB). GTX60495

GTX60495 WB Image

WB analysis of A431 (1), MCF-7 (2), Jurkat (3), HeLa (4), HepG2 (5), A549 (6), NIH3T3 (7), Cos7 (8) and PC-12 (9) cell lysate using GTX60495 Prohibitin antibody [5H7].

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339