APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.2-2.5 ug/ml |
| 2-10 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
293T , Daudi , human B lymphoblast , Jurkat
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a C-terminal region of Human Prohibitin
Purification
Affinity Purified
Conjugation
Unconjugated
RRID
AB_424791
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
prohibitin , HEL-215 , HEL-S-54e , PHB1
Cellular Localization
Mitochondrion inner membrane
Background
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
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GTX77843 IHC-P Image
IHC-P analysis of human bronchial epithelial tissue using GTX77843 Prohibitin antibody at 1:600. Left to right:DAPI, PHB, Merge.
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GTX77843 IHC-P Image
IHC-P analysis of human liver tissue using GTX77843 Prohibitin antibody at 1:600. Left to right:DAPI, PHB, Merge.
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GTX77843 IHC-P Image
IHC-P analysis of human stomach tissue using GTX77843 Prohibitin antibody at 4.0-8.0μg/ml.
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GTX77843 IHC-P Image
IHC-P analysis of human lung tissue using GTX77843 Prohibitin antibody at 1:600. Left to right:DAPI, PHB, Merge.
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GTX77843 WB Image
WB analysis of Daudi cells using GTX77843 Prohibitin antibody at 1μg/ml.
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GTX77843 WB Image
WB analysis of 293T cells using GTX77843 Prohibitin antibody at 1μg/ml.
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GTX77843 WB Image
WB analysis of Jurkat cells using GTX77843 Prohibitin antibody at 0.2-1μg/ml.
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GTX77843 WB Image
WB analysis of human B lymphoblast cells using GTX77843 Prohibitin antibody at 1μg/ml.
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GTX77843 WB Image
WB analysis of 293T cells using GTX77843 Prohibitin antibody at 1.0μg/ml.
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REFERENCE
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REVIEW
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