APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
IMR32 , U87-MG , PC-12 , Rat2 , Neuro2A , GL261 , C8D30
Predict Reactivity
Zebrafish, Bovine, Cat, Dog, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.91 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10723105
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
prohibitin , HEL-215 , HEL-S-54e , PHB1
Cellular Localization
Mitochondrion inner membrane
Background
Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113243 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Prohibitin(GTX113243) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113243 WB Image
Prohibitin antibody detects PHB protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
12 % SDS-PAGE
Prohibitin antibody (GTX113243) dilution: 1:1000
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GTX113243 WB Image
Prohibitin antibody detects PHB protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
Prohibitin antibody (GTX113243) dilution: 1:1000
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GTX113243 IHC-P Image
Prohibitin antibody detects Prohibitin protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Prohibitin antibody (GTX113243) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113243 ICC/IF Image
Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: Prohibitin protein stained by Prohibitin antibody (GTX113243) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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GTX113243 IHC-P Image
Prohibitin antibody detects Prohibitin protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Prohibitin antibody (GTX113243) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113243 WB Image
Sample (30 ug of whole cell lysate)
A: IMR32
B: U87-MG
12% SDS PAGE
GTX113243 diluted at 1:1000
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REFERENCE
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REVIEW
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