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Prohibitin antibody

Anti-Prohibitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65815
Anti-Prohibitin antibody used in Western Blot (WB). GTX65815

Cat. No. GTX65815

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all Prohibitin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 73-272 of human PHB (NP_002625.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prohibitin , HEL-215 , HEL-S-54e , PHB1

Cellular Localization

Mitochondrion inner membrane

Background

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-Prohibitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65815

GTX65815 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX65815 Prohibitin antibody.
Blue : DAPI

Anti-Prohibitin antibody used in Western Blot (WB). GTX65815

GTX65815 WB Image

WB analysis of various sample lysates using GTX65815 Prohibitin antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399