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Prohibitin (phospho Thr258) antibody

Anti-Prohibitin (phospho Thr258) antibody used in Western Blot (WB). GTX55299

Cat. No. GTX55299

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Prohibitin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1: 500-1:1000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 258 (N-I-T(p)-Y-L) derived from human Prohibitin.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_2887922

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prohibitin , HEL-215 , HEL-S-54e , PHB1

Cellular Localization

Mitochondrion inner membrane

Background

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-Prohibitin (phospho Thr258) antibody used in Western Blot (WB). GTX55299

GTX55299 WB Image

WB analysis of extracts from MCF, PC12, 293, and 3T3 cells using GTX55299 Prohibitin (phospho Thr258) antibody.

REFERENCE

Application Reference

Li XH et al. Asian J Androl 2020; 22 (6) : 583-589 Prohibitin (PHB) interacts with AKT in mitochondria to coordinately modulate sperm motility.
Application : WB, ICC/IF
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399