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Prolactin Receptor antibody [U5]

Anti-Prolactin Receptor antibody [U5] used in IHC (Frozen sections) (IHC-Fr). GTX22772
Anti-Prolactin Receptor antibody [U5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22772
Anti-Prolactin Receptor antibody [U5] used in IHC (Paraffin sections) (IHC-P). GTX22772
Anti-Prolactin Receptor antibody [U5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22772
Anti-Prolactin Receptor antibody [U5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22772

Cat. No. GTX22772

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 U5

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Rabbit, Bovine, Hamster, Pig, Horse, Primate
Package
100 μg ($399)
See all Prolactin Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 5 μg/ml
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Product Note

This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Purified rat liver PRL receptor.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prolactin receptor , RATPRLR

Cellular Localization

Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-Prolactin Receptor antibody [U5] used in IHC (Frozen sections) (IHC-Fr). GTX22772

GTX22772 IHC-Fr Image

IHC-Fr analysis of rat Islets of langerhans using GTX22772 Prolactin Receptor antibody [U5].

Anti-Prolactin Receptor antibody [U5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22772

GTX22772 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX22772 Prolactin Receptor antibody [U5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Prolactin Receptor antibody [U5] used in IHC (Paraffin sections) (IHC-P). GTX22772

GTX22772 IHC-P Image

IHC-P analysis of mammary gland tissue from lactating light horse mare using GTX22772 Prolactin Receptor antibody [U5].
Antigen retireval : 95ºC for 20 min in TrizmaR buffer (pH 10.0)
Dilution : 1:100

Anti-Prolactin Receptor antibody [U5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22772

GTX22772 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX22772 Prolactin Receptor antibody [U5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-Prolactin Receptor antibody [U5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22772

GTX22772 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX22772 Prolactin Receptor antibody [U5]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

Application Reference

Nguyen TD et al. Int J Cancer 2020; (Epub) Tumor-secreted proliferin-1 regulates adipogenesis and lipolysis in cachexia.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399