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Pronase reagent

Cat No. GTX73181

Application IHC
Package
100 ml ($179)
PRODUCT

Product Description

Pronase Reagent
APPLICATION

Application Note

For IHC: Use as antigen retriever for some antibodies in IHC. Reagent is supplied in ready-to-use format. Over-digestion may detach tissue sections from slides or may result in the loss of tissue morphology. It may be necessary to use + charge, sialanized or polylysine coated slides.
PROPERTIES

Form

Liquid

Storage

Keep as concentrated solution, aliquot and store at 4ºC. Do not freeze.

Concentration

Ready-to-use

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Pronase is used for photolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
REFERENCE
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Package List Price ($)
$ 179