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Prorenin antibody [4B5-E3] (Inhibitory)

Cat. No. GTX79679

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B5-E3

Isotype

 IgG2b

Application

 WB, ELISA, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($399)
See all Renin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Product Note

Recognises amino acid residues 3-43 (MARLGPEWSQPMKR) of the prosegment of prorenin.

PROPERTIES

Form

Liquid

Buffer

0.05M Sodium Phosphate, 0.1M NaCl, 1mM EDTA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length wild-type human protein (Glycosylated).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11165876

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HNFJ2,REN,renin,Renin,Prorenin

Cellular Localization

Secreted,Membrane

Background

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Application Reference

Burdman I et al. Rapid Commun Mass Spectrom 2020; 34 (24) : e8932 Human prorenin determination by hybrid immunocapture liquid chromatography/mass spectrometry: A mixed-solvent-triggered digestion utilizing D-optimal design.
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REVIEW

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Package List Price ($)
$ 399