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Prostaglandin E Receptor EP2 antibody [GT1308]

Recombinant antibody
Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in Western Blot (WB). GTX03220
Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in IHC (Paraffin sections) (IHC-P). GTX03220
Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03220
Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in Western Blot (WB). GTX03220
Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03220
Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03220

Cat. No. GTX03220

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1308

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all Prostaglandin E Receptor EP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Observed MW

52 kDa. The observed M.W. is based on the following publication. PMID: 23048027

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PTGER2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prostaglandin E receptor 2 , EP2

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a receptor for prostaglandin E2, a metabolite of arachidonic acid which has different biologic activities in a wide range of tissues. Mutations in this gene are associated with aspirin-induced susceptibility to asthma. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in Western Blot (WB). GTX03220

GTX03220 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Prostaglandin E Receptor EP2 antibody [GT1308] (GTX03220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 23048027

Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in IHC (Paraffin sections) (IHC-P). GTX03220

GTX03220 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX03220 Prostaglandin E Receptor EP2 antibody [GT1308].
Dilution : 1:100

Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03220

GTX03220 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX03220 Prostaglandin E Receptor EP2 antibody [GT1308].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in Western Blot (WB). GTX03220

GTX03220 WB Image

WB analysis of various samples using GTX03220 Prostaglandin E Receptor EP2 antibody [GT1308].
Dilution : 1:1000
Loading : 25μg per lane

Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03220

GTX03220 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX03220 Prostaglandin E Receptor EP2 antibody [GT1308].
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-Prostaglandin E Receptor EP2 antibody [GT1308] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03220

GTX03220 ICC/IF Image

ICC/IF analysis of C6 cells using GTX03220 Prostaglandin E Receptor EP2 antibody [GT1308].
Blue : DAPI for nuclear staining
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 439