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Prostasin antibody [N1C2]

Anti-Prostasin antibody [N1C2] used in Western Blot (WB). GTX103976
Anti-Prostasin antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103976

Cat. No. GTX103976

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all Prostasin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HeLaS3 , Raji

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Prostasin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951483

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine protease 8 , CAP1 , PROSTASIN

Cellular Localization

Prostasin: Cell membrane; Single-pass membrane protein , Prostasin light chain: Secreted , extracellular space , Prostasin heavy chain: Secreted

Background

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Prostasin antibody [N1C2] used in Western Blot (WB). GTX103976

GTX103976 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
B:Raji (GTX27908)
10% SDS PAGE
GTX103976 diluted at 1:1000

Anti-Prostasin antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103976

GTX103976 IHC-P Image

Prostasin antibody [N1C2] detects Prostasin protein at cytosol on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
Prostasin antibody [N1C2] (GTX103976) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Higashi T et al. J Cell Biol 2023; 222 (1) EpCAM proteolysis and release of complexed claudin-7 repair and maintain the tight junction barrier.
Drees L et al. PLoS Genet 2019; 15 (1) : e1007882 Conserved function of the matriptase-prostasin proteolytic cascade during epithelial morphogenesis.
Application : IHC-Wm
Reactivity : Drosophila
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169