GeneTex
United States (US)

Prostatic Acid Phosphatase antibody, Internal

GTX88063_967_IHC-P_18121411_904.jpg
GTX88063_2344_WB_18121411_579.jpg

Cat No. GTX88063

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 3-5μg/ml

Note :

IHC-P
Human Prostate shows cytoplasm staining in some secretory cells of the gland.

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88063-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-YGIHKQKEKSRLQ, from the internal region of the protein sequence according to NP_001090.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Acid Phosphatase, Prostate,5'-Nt,Acp-3,Acp3,Acpp

Background

This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]

Database

DATA IMAGES
GTX88063_967_IHC-P_18121411_904.jpg

GTX88063 IHC-P Image

IHC-P analysis of human prostate using GTX88063 Prostatic Acid Phosphatase antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88063_2344_WB_18121411_579.jpg

GTX88063 WB Image

WB analysis of human prostate lysate using GTX88063 Prostatic Acid Phosphatase antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329