PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Prostate: A strong PSAP immunostaining should, be seen in all glandular cells.
Negative Control
Appendix: All epithelial and non-epithelial structures must not show any PSAP immunostaining.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human ACPP protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
acid phosphatase, prostate , 5'-NT , ACP-3 , ACP3
Cellular Localization
Secreted , Cell membrane , Lysosome membrane
Background
This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]
Database
Research Area
DATA IMAGES
![Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396](/templates/default/js/lightbox/images/loading.gif "Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396")
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GTX04396 IHC-P Image
IHC-P analysis of human prostate tissue using GTX04396 Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™.
Strong prostatic acid phosphatase immunostaining in all acinar epithelial cells of the prostate.
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GTX04396 IHC-P Image
IHC-P analysis of human prostatic adenocarcinoma (PAC) tissue with (Gleason 4+4=8) tissue using GTX04396 Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™.
Prostatic adenocarcinoma (Gleason 4+4=8) with strong diffuse PSAP positivity.
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GTX04396 IHC-P Image
IHC-P analysis of human mucosa from appendix tissue using GTX04396 Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™.
Complete absence of prostatic acid phosphatase immunostaining in all epithelial and non-epithelial cell types of the appendix.
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REFERENCE
There are currently no references for Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ (GTX04396). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ (GTX04396). Be the first to share your experience with this product.
![Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396](/upload/website/prouct_img/small/GTX04396/GTX04396_20230728_IHC-P_102_23072722_589.webp "Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396")
![Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396](/upload/website/prouct_img/small/GTX04396/GTX04396_20230728_IHC-P_232_23072723_804.webp "Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396")
![Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396](/upload/website/prouct_img/small/GTX04396/GTX04396_20230728_IHC-P_362_23072723_611.webp "Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396")
![Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396](/upload/website/prouct_img/normal/GTX04396/GTX04396_20230728_IHC-P_102_23072722_589.webp "Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396")
![Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396](/upload/website/prouct_img/normal/GTX04396/GTX04396_20230728_IHC-P_232_23072723_804.webp "Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396")
![Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396](/upload/website/prouct_img/normal/GTX04396/GTX04396_20230728_IHC-P_362_23072723_611.webp "Anti-Prostatic Acid Phosphatase antibody [MSVA-452M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04396")