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United States (US)

Proteasome 20S alpha 1 antibody [N1C3]

GTX104373_40079_WB_M_brain_19011506_315.jpg
GTX104373_IHC_19011506_412.jpg
GTX104373_40422_WB_19011506_503.jpg
GTX104373_WB_19011506_338.jpg
GTX104373_IFA_19011506_566.jpg
GTX104373_40079_20150506_IHC_R_19011506_781.jpg

Cat No. GTX104373

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , U87-MG , MCF-7 , mouse brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.71mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HC2 antibody, MGC14542 antibody, MGC14575 antibody, MGC14751 antibody, MGC1667 antibody, MGC21459 antibody, MGC22853 antibody, MGC23915 antibody, NU antibody, PROS30 antibody, PSMA1 antibody, proteasome nu chain antibody, protein P30-33K antibody, macropain subunit nu antibody, PROS-30 antibody, macropain subunit C2 antibody, multicatalytic endopeptidase complex subunit C2 antibody, "proteasome subunit, alpha-type, 1 antibody", proteasome component C2 antibody, proteasome subunit nu antibody, proteasome subunit alpha type-1 antibody, 30 kDa prosomal protein antibody, "proteasome (prosome, macropain) subunit, alpha type, 1 antibody"

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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Database

Research Area

DATA IMAGES
GTX104373_40079_WB_M_brain_19011506_315.jpg

GTX104373 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX104373 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104373_IHC_19011506_412.jpg

GTX104373 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using 20S Proteasome alpha1(GTX104373) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104373_40422_WB_19011506_503.jpg

GTX104373 WB Image

Sample (30 µg of whole cell lysate)
A: U87-MG
B: MCF-7
12% SDS PAGE
GTX104373 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104373_WB_19011506_338.jpg

GTX104373 WB Image

Sample (30 µg of whole cell lysate)
A: Raji
12% SDS PAGE
GTX104373 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104373_IFA_19011506_566.jpg

GTX104373 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha1(GTX104373) antibody at 1:200 dilution.

GTX104373_40079_20150506_IHC_R_19011506_781.jpg

GTX104373 IHC-P Image

Proteasome 20S alpha 1 antibody [N1C3] detects Proteasome 20S alpha 1 protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
Proteasome 20S alpha 1 antibody [N1C3] (GTX104373) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf