APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji , Mouse brain , U87-MG , MCF-7
Predict Reactivity
Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.71 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951503
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proteasome subunit alpha 1 , HC2 , HEL-S-275 , NU , PROS30
Cellular Localization
Cytoplasm , Nucleus
Background
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Database
Research Area
DATA IMAGES
|
GTX104373 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using 20S Proteasome alpha1(GTX104373) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX104373 WB Image
Sample (30 μg of whole cell lysate) A: Raji 12% SDS PAGE GTX104373 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX104373 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha1(GTX104373) antibody at 1:200 dilution.
|
|
GTX104373 WB Image
Sample (30 μg of whole cell lysate) A: U87-MG B: MCF-7 12% SDS PAGE GTX104373 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX104373 IHC-P Image
Proteasome 20S alpha 1 antibody [N1C3] detects Proteasome 20S alpha 1 protein at cytoplasm in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. Proteasome 20S alpha 1 antibody [N1C3] (GTX104373) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX104373 WB Image
Sample (50 μg of whole cell lysate) A: Mouse brain 12% SDS PAGE GTX104373 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for Proteasome 20S alpha 1 antibody [N1C3] (GTX104373). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Proteasome 20S alpha 1 antibody [N1C3] (GTX104373). Be the first to share your experience with this product.