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Proteasome 20S alpha 1 antibody [N1C3]

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104373
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104373
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104373

Cat No. GTX104373

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , U87-MG , MCF-7 , mouse brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

proteasome subunit alpha 1 , HC2 , HEL-S-275 , NU , PROS30

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Database

Research Area

DATA IMAGES
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373

GTX104373 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX104373 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104373

GTX104373 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using 20S Proteasome alpha1(GTX104373) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373

GTX104373 WB Image

Sample (30 µg of whole cell lysate)
A: U87-MG
B: MCF-7
12% SDS PAGE
GTX104373 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373

GTX104373 WB Image

Sample (30 µg of whole cell lysate)
A: Raji
12% SDS PAGE
GTX104373 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104373

GTX104373 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha1(GTX104373) antibody at 1:200 dilution.

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104373

GTX104373 IHC-P Image

Proteasome 20S alpha 1 antibody [N1C3] detects Proteasome 20S alpha 1 protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
Proteasome 20S alpha 1 antibody [N1C3] (GTX104373) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169