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Proteasome 20S alpha 1 antibody [N1C3]

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104373
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104373
Anti-Proteasome 20S alpha 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104373

Cat No. GTX104373

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , U87-MG , MCF-7 , mouse brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951503

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proteasome subunit alpha 1 , HC2 , HEL-S-275 , NU , PROS30

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Database

Research Area

DATA IMAGES

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373

GTX104373 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX104373 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104373

GTX104373 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using 20S Proteasome alpha1(GTX104373) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373

GTX104373 WB Image

Sample (30 μg of whole cell lysate)
A: U87-MG
B: MCF-7
12% SDS PAGE
GTX104373 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Western Blot (WB). GTX104373

GTX104373 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
12% SDS PAGE
GTX104373 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104373

GTX104373 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha1(GTX104373) antibody at 1:200 dilution.

Anti-Proteasome 20S alpha 1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104373

GTX104373 IHC-P Image

Proteasome 20S alpha 1 antibody [N1C3] detects Proteasome 20S alpha 1 protein at cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
Proteasome 20S alpha 1 antibody [N1C3] (GTX104373) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169