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Proteasome 20S alpha 1 antibody

Anti-Proteasome 20S alpha 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32814
Anti-Proteasome 20S alpha 1 antibody used in Western Blot (WB). GTX32814

Cat No. GTX32814

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($329)
See all Proteasome 20S alpha 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:10-1:100
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human PSMA1 (NP_002777.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proteasome subunit alpha 1 , HC2 , HEL-S-275 , NU , PROS30

Cellular Localization

Cytoplasm,Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES

Anti-Proteasome 20S alpha 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32814

GTX32814 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32814 Proteasome 20S alpha 1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Proteasome 20S alpha 1 antibody used in Western Blot (WB). GTX32814

GTX32814 WB Image

WB analysis of various sample lysates using GTX32814 Proteasome 20S alpha 1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329