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Proteasome 20S alpha 2 antibody [N1C3]

Anti-Proteasome 20S alpha 2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103620
Anti-Proteasome 20S alpha 2 antibody [N1C3] used in Western Blot (WB). GTX103620
Anti-Proteasome 20S alpha 2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103620

Cat. No. GTX103620

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Proteasome 20S alpha 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951505

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome subunit alpha 2 , HC3 , MU , PMSA2 , PSC2

Cellular Localization

Cytoplasm , Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Proteasome 20S alpha 2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103620

GTX103620 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha2(GTX103620) antibody at 1:200 dilution.

Anti-Proteasome 20S alpha 2 antibody [N1C3] used in Western Blot (WB). GTX103620

GTX103620 WB Image

Sample(30μg of whole cell lysate)
A: H1299
B: HeLa S3 (GTX14654)
C: Hep G2 (GTX27900)
D: MOLT4 (GTX27912)
E: Raji (GTX27908)
12% SDS PAGE
GTX103620 diluted at 1:1000

Anti-Proteasome 20S alpha 2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103620

GTX103620 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using Proteasome 20S alpha 2(GTX103620) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Taniguchi H et al. Biochem Biophys Res Commun 2017; 484 (1) : 176-183 Possible roles of the transcription factor Nrf1 (NFE2L1) in neural homeostasis by regulating the gene expression of deubiquitinating enzymes.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169