Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Proteasome 20S beta 7 antibody [N2C3] (GTX117690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using Proteasome 20S beta 7(GTX117690) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Proteasome 20S beta 7(GTX117690) antibody at 1:200 dilution.

Proteasome 20S beta 7 antibody [N2C3] detects Proteasome 20S beta 7 protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Proteasome 20S beta 7 antibody [N2C3] (GTX117690) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX117690 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.