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Proteasome 26S S3 antibody [C1C3]

Anti-Proteasome 26S S3 antibody [C1C3] used in Western Blot (WB). GTX102291
Anti-Proteasome 26S S3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102291

Cat. No. GTX102291

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Proteasome 26S S3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 26S S3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951519

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome 26S subunit, non-ATPase 3 , P58 , RPN3 , S3 , TSTA2

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Proteasome 26S S3 antibody [C1C3] used in Western Blot (WB). GTX102291

GTX102291 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
D: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX102291 diluted at 1:1000

Anti-Proteasome 26S S3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102291

GTX102291 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Proteasome 26S S3(GTX102291) antibody at 1:200 dilution.

REFERENCE

Application Reference

Rao KN et al. Sci Rep 2015; 5 : 11137 Ablation of retinal ciliopathy protein RPGR results in altered photoreceptor ciliary composition.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169