*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.97 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human Proteasome 26S S3. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
proteasome 26S subunit, non-ATPase 3 , P58 , RPN3 , S3 , TSTA2
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. [provided by RefSeq]
GTX102291 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
D: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX102291 diluted at 1:1000
GTX102291 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using Proteasome 26S S3(GTX102291) antibody at 1:200 dilution.