GeneTex
United States (US)

Proteasome 26S S3 antibody [N1N3]

GTX102290_39694_WB_19011506_129.jpg
GTX102290_WB_M_19011506_933.jpg
GTX102290_IHC_19011506_428.jpg

Cat No. GTX102290

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

A549 , Hela , Jurkat , NT2D1 , NIH-3T3

Predict Reactivity

Rat, Bovine, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Proteasome 26S S3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proteasome 26S Subunit, Non-Atpase 3,P58,Rpn3,S3,Tsta2,Psmd3

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102290_39694_WB_19011506_129.jpg

GTX102290 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX102290 diluted at 1:500

GTX102290_WB_M_19011506_933.jpg

GTX102290 WB Image

Sample (30 ug of whole cell lysate)

A: NIH-3T3

7.5% SDS PAGE

Proteasome 19S S3 antibody

GTX102290 diluted at 1:1000

GTX102290_IHC_19011506_428.jpg

GTX102290 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Proteasome 26S S3(GTX102290) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169