APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , H1299 , HeLa
Predict Reactivity
Mouse, Rat, Rabbit, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.73 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Proteasome beta 4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037786
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
proteasome subunit beta 4 , HN3 , HsN3 , PRAAS3 , PROS-26 , PROS26
Cellular Localization
Cytoplasm , Nucleus
Background
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Proteasome beta 4 antibody [N1C3] used in Western Blot (WB). GTX101780](/upload/website/prouct_img/middle/GTX101780/GTX101780_WB_19011506_778.jpg "Anti-Proteasome beta 4 antibody [N1C3] used in Western Blot (WB). GTX101780")
|
GTX101780 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX101780 diluted at 1:1000
|
REFERENCE
There are currently no references for Proteasome beta 4 antibody [N1C3] (GTX101780). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Proteasome beta 4 antibody [N1C3] (GTX101780). Be the first to share your experience with this product.
![Anti-Proteasome beta 4 antibody [N1C3] used in Western Blot (WB). GTX101780](/upload/website/prouct_img/small/GTX101780/GTX101780_WB_19011506_778.jpg "Anti-Proteasome beta 4 antibody [N1C3] used in Western Blot (WB). GTX101780")
![Anti-Proteasome beta 4 antibody [N1C3] used in Western Blot (WB). GTX101780](/upload/website/prouct_img/normal/GTX101780/GTX101780_WB_19011506_778.jpg "Anti-Proteasome beta 4 antibody [N1C3] used in Western Blot (WB). GTX101780")
![Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624](/upload/website/prouct_img/small/GTX108624/GTX108624_43754_20191108_WB_KO_watermark_20092220_924.jpg "Anti-Cyclin D1 antibody [N1C3] used in Western Blot (WB). GTX108624")
