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Protein A (FITC)

Cat. No. GTX30958

Application

 ICC/IF, FACS

Species

 Staphylococcus aureus
Package
1 ml ($149)
See all Protein A products
PRODUCT

Summary

Protein A (MW 42 kDa) is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. One protein A molecule has been shown to bind at least 2 molecules of IgG simultaneously. The IgG binding domain of Protein A consists of three anti-parallel α-helicies, the third of which is disrupted when the protein is complexed with the Fc region of the immunoglobulins. Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a, and IgG2b. Protein A also binds IgGs from other species, including monkey, rabbit, pig, guinea pig, dog, and cat. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm, Fluorophore/Protein: A492nm/A280nm=2.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM Phosphate, 150mM NaCl, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Region/Sequence

Native Protein

Expression System

Native Protein

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Protein A (MW 42 kDa) is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. One protein A molecule has been shown to bind at least 2 molecules of IgG simultaneously. The IgG binding domain of Protein A consists of three anti-parallel α-helicies, the third of which is disrupted when the protein is complexed with the Fc region of the immunoglobulins. Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a, and IgG2b. Protein A also binds IgGs from other species, including monkey, rabbit, pig, guinea pig, dog, and cat. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm, Fluorophore/Protein: A492nm/A280nm=2.

Research Area

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 149