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Protein A antibody (Biotin)

Anti-Protein A antibody (Biotin) used in ELISA (ELISA). GTX77597
Anti-Protein A antibody (Biotin) used in Western Blot (WB). GTX77597

Cat. No. GTX77597

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ELISA

Reactivity

 Staphylococcus aureus
Package
1 ml ($399)
See all Protein A products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1000-1:10000.
For ELISA: Use at a dilution of 1:5000-1:40000.
Optimal dilutions should be determined experimentally by the researcher.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Purified recombinant protein A from Staphylococcus aureus.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Biotin

RRID

AB_379849

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Protein A

Research Area

DATA IMAGES

Anti-Protein A antibody (Biotin) used in ELISA (ELISA). GTX77597

GTX77597 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Protein A protein (0.2 μg/mL). Coated protein probed with Protein A antibody (Biotin) (GTX127324-02) (14-10000 pg/mL). The peroxidase streptavidin secondary antibody (1:10000) detected bound primary antibody.

Anti-Protein A antibody (Biotin) used in Western Blot (WB). GTX77597

GTX77597 WB Image

Protein A antibody detects Protein A protein by western blot analysis. Whole cell extracts (0.1ng and 0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Protein A antibody (GTX77597) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 399