GeneTex
United States (US)

Protein A antibody (Biotin)

GTX77597_41885_20141219_ELISA_18121411_644.jpg
GTX77597_41885_20150119_WB_18121411_118.jpg

Cat No. GTX77597

Host Chicken
Clonality Polyclonal
Application WB, ELISA
APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:1000-1:10000.
For ELISA: Use at a dilution of 1:5000-1:40000.
Optimal dilutions should be determined experimentally by the researcher.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Purified recombinant protein A from Staphylococcus aureus.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein A
DATA IMAGES
GTX77597_41885_20141219_ELISA_18121411_644.jpg

GTX77597 ELISA Image

An ELISA plate is coated with 50 μL of a Protein A recombinant protein at 10 μg/mL. The coated protein is detected with Biotinylated anti-Protein A antibody (GTX77597) at concentration ranged from 0.24 to 250 ng/mL

GTX77597_41885_20150119_WB_18121411_118.jpg

GTX77597 WB Image

Protein A antibody detects Protein A protein by western blot analysis. Whole cell extracts (0.1ng and 0.1 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Protein A antibody (GTX77597) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Package List Price ($)
$ 239