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Protein A antibody (HRP)

Anti-Protein A antibody (HRP) used in Western Blot (WB). GTX77595
Anti-Protein A antibody (HRP) used in ELISA (ELISA). GTX77595

Cat. No. GTX77595

Reference 1

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB, ELISA

Reactivity

 Staphylococcus aureus
Package
1 ml ($399)
See all Protein A products

APPLICATION

Application Note

For ELISA: Use at a dilution of 1:5,000-1:20,000. For WB: Use at a dilution of 1:1,000-1:20,000. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

Protein A

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

Chickens were immunized with purified recombinant protein A. After multiple immunizations in Freund's adjuvant, eggs were collected and delipidated. Antibodies were immunoaffinity purified off protein A agarose and conjugated with horseradish peroxidase (HRP).

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_379859

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Protein A antibody (HRP) used in Western Blot (WB). GTX77595

GTX77595 WB Image

Protein A antibody detects Protein A protein by Western blot analysis. Recombinant Protein (0.1ng and 0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Protein A antibody (GTX77595) at a dilution of 1:20000.

Anti-Protein A antibody (HRP) used in ELISA (ELISA). GTX77595

GTX77595 ELISA Image

An ELISA plate is coated with 50 μL of a Protein A recombinant protein at 10 μg/mL. The coated protein is detected with HRP-conjugated anti-Protein A antibody (GTX77595) at concentration ranged from 0.49 to 500 ng/mL.

REFERENCE

Application Reference

Fukuda T et al. Mol Cell 2023; 83 (12) The mitochondrial intermembrane space protein mitofissin drives mitochondrial fission required for mitophagy
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SDS
Proclin.pdf
Package List Price ($)
$ 399