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Protein A antibody

Anti-Protein A antibody used in Western Blot (WB). GTX127324

Cat. No. GTX127324

1

Host

 Chicken

Clonality

 Polyclonal

Isotype

 IgY

Application

 WB
Package
100 μl ($399),
25 μl ($169)
See all Protein A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
Not tested in other applications.

Positive Control

0.1 ng protein A

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Protein A

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885641

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Protein A (S.aureus)

Research Area

DATA IMAGES

Anti-Protein A antibody used in Western Blot (WB). GTX127324

GTX127324 WB Image

Western blot analysis of 0.1 ng protein A, using protein A antibody(GTX127324) (A) and GFP antibody(GTX124117) (B as negative control). After incubation of primary antibody, Easy blocker (GTX425858) was used to block interaction of protein A with secondary antibody.
A: 1 mg/ml IgY of Protein A (1:10000).
B: 1 mg/ml IgY of GFP (1:10000).

REFERENCE

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REVIEW

Protein A antibody Cat. No. GTX127324

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Protein A antibody used in Western Blot (WB). GTX127324
Date : Anonymous submitted on 10-Aug-2018
Score :
Application Tested : WB
Sample : Fission yeast
Amount used : 10 ul of culture supernatant or 10 μg of cell lysate
Blocking : 5% skim milk, 26°C, 1Hr
Primary Antibody : 1:5000, 26°C, 3Hr
Lane Description : Lane 1: 972 (S. prombe WT) sup., Lane 2: 972 Ptr8p-Myc and Sec5p-FLAG tagged sup., Lane 3: 972 Ptr8p-FLAG and Cid12p-TAP tagged sup., Lane 4: 972 Ptr8p-Myc and Cid12p-TAP tagged whole cell lysate, Lane 5: 972 Ptr8p-Myc and Cid12p-TAP tagged sup., Lane 6: 972 Ptr8p-Myc and Cid12p-TAP tagged precipitation, Lane 7: 972 Ptr8p-Myc and Cid12p-TAP tagged sup., Lane 8: 972 Ptr8p-Myc and Cid12p-TAP tagged sup.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169