GeneTex
United States (US)

Protein A antibody

GTX127324_40926_WB_18121410_506.jpg

Cat No. GTX127324

Host Chicken
Clonality Polyclonal
Isotype IgY
Application WB
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
Not tested in other applications.

Positive Control

0.1 ng protein A
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Protein A

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein A (S.aureus)
DATA IMAGES
GTX127324_40926_WB_18121410_506.jpg

GTX127324 WB Image

Western blot analysis of 0.1 ng protein A, using protein A antibody(GTX127324) (A) and GFP antibody(GTX124117) (B as negative control). After incubation of primary antibody, Easy blocker (GTX425858) was used to block interaction of protein A with secondary antibody.
A: 1 mg/ml IgY of Protein A (1:10000).
B: 1 mg/ml IgY of GFP (1:10000).

REVIEW

Protein A antibody Cat No. GTX127324

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2018
Score :
Application Tested : WB
Sample : Fission yeast
Amount used : 10 ul of culture supernatant or 10 μg of cell lysate
Blocking : 5% skim milk, 26°C, 1Hr
Primary Antibody : 1:5000, 26°C, 3Hr
Lane Description : Lane 1: 972 (S. prombe WT) sup., Lane 2: 972 Ptr8p-Myc and Sec5p-FLAG tagged sup., Lane 3: 972 Ptr8p-FLAG and Cid12p-TAP tagged sup., Lane 4: 972 Ptr8p-Myc and Cid12p-TAP tagged whole cell lysate, Lane 5: 972 Ptr8p-Myc and Cid12p-TAP tagged sup., Lane 6: 972 Ptr8p-Myc and Cid12p-TAP tagged precipitation, Lane 7: 972 Ptr8p-Myc and Cid12p-TAP tagged sup., Lane 8: 972 Ptr8p-Myc and Cid12p-TAP tagged sup.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169