GeneTex
United States (US)

Protein C antibody

GTX105844_40611_IHC_19011506_282.jpg
GTX105844_40611_IFA_19011506_423.jpg
GTX105844_40611_WB_19011506_522.jpg

Cat No. GTX105844

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HepG2 , HCT116

Predict Reactivity

Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Protein C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APC antibody, PC antibody, PROC1 antibody, PROC antibody, vitamin K-dependent protein C antibody, anticoagulant protein C antibody, blood coagulation factor XIV antibody, autoprothrombin IIA antibody, protein C (inactivator of coagulation factors Va and VIIIa) antibody

Background

The PROC gene encodes protein C (EC 3.4.21.69), a vitamin K-dependent plasma glycoprotein that is a key component of the anticoagulant system. Protein C is cleaved to its activated form, 'activated protein C' (APC) on endothelial cells by the thrombin-thrombomodulin complex (MIM 176930; MIM 188040) and then acts as a serine protease to degrade the activated forms of coagulation factors V (F5; MIM 612309) and VIII (F8; see MIM 306700). Protein S (PROS1; MIM 176880), also a vitamin K-dependent plasma protein, functions as a cofactor to activated protein C.[supplied by OMIM]
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Database

DATA IMAGES
GTX105844_40611_IHC_19011506_282.jpg

GTX105844 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Protein C(GTX105844) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105844_40611_IFA_19011506_423.jpg

GTX105844 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Protein C(GTX105844) antibody at 1:500 dilution.

GTX105844_40611_WB_19011506_522.jpg

GTX105844 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
10% SDS PAGE
GTX105844 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169