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United States (US)

Protein G (FITC)

Cat. No. GTX30959

Application

 ICC/IF, FACS

Species

 Streptococcus
Package
1 ml ($219)
See all Protein G products
PRODUCT

Summary

Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm, Fluorophore/Protein: A492nm/A280nm

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM Phosphate, 150mM NaCl, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Recombinant Protein G (FITC)

Expression System

E. coli

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm, Fluorophore/Protein: A492nm/A280nm

Research Area

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 219