PRODUCT
Product Description
Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm, Fluorophore/Protein: A492nm/A280nm
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
10 mM phosphate, 150 mM NaCl, 1% BSA and 0.05% sodium azide.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Conjugation
Fluorescein isothiocyanate (FITC)
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Background
Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm, Fluorophore/Protein: A492nm/A280nm
