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Protein G (FITC)

Cat No. GTX30959

Application ICC/IF, FACS
Package
1 ml ($219)
PRODUCT

Product Description

Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm, Fluorophore/Protein: A492nm/A280nm
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

10 mM phosphate, 150 mM NaCl, 1% BSA and 0.05% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from recombinant forms of Protein G. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm, Fluorophore/Protein: A492nm/A280nm
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Package List Price ($)
$ 219