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Protein G antibody (FITC)

Anti-Protein G antibody (FITC) used in Dot blot (Dot). GTX27249
Anti-Protein G antibody (FITC) used in Dot blot (Dot). GTX27249

Cat. No. GTX27249

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, FACS, Dot, ELISA

Reactivity

 Staphylococcus aureus
Package
1 mg ($399)
See all Protein G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:500-1:2500
IHC-Fr Assay dependent
FACS Assay dependent
Dot Assay dependent
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Staphylococcus aureus

Immunogen

Protein G collected from Streptococcus sp

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Fluorescein isothiocyanate (FITC)
Ratio : 4.5 molecules FITC per Rabbit IgG molecule.

RRID

AB_385372

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Protein G antibody (FITC) used in Dot blot (Dot). GTX27249

GTX27249 Dot Image

Dot Blot of Protein G Antibody Fluorescein Conjugated (GTX27249). Antigen 1: Human IgG. Antigen 2: Rat IgG. Antigen 3: Dog IgG. Load: Starting dot is 200 ng followed by a 3-fold serial dilution. Primary antibody: Protein G at 1:1000 for 60 min RT. Secondary antibody: Fluorescein rabbit anti-Protein G at 1:1,000 for 30 min at RT. Block: blocking buffer for 30 min at RT.

Anti-Protein G antibody (FITC) used in Dot blot (Dot). GTX27249

GTX27249 Dot Image

Dot blot analysis of various samples using GTX27249 Protein G antibody (FITC).
Row 1 : Human IgG
Row 2 : Rat IgG
Row 3 : Dog IgG
Dilution : 1:1000

REFERENCE

Application Reference

Iwata H et al. J Pathol 2018; (Epub) Fc-binding proteins enhance autoantibody-induced BP180 depletion in pemphigoid.
Application : IHC-Fr
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Package List Price ($)
$ 399