GeneTex
  • Country / Location Selection

United States (US)

Proteosome Activator, 200 kDa antibody

Anti-Proteosome Activator, 200 kDa antibody used in Western Blot (WB). GTX25620
Anti-Proteosome Activator, 200 kDa antibody used in Western Blot (WB). GTX25620

Cat No. GTX25620

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat, Bovine
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:250
ICC/IF 1:600
IP Assay dependent
Not tested in other applications.

Calculated MW

211 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues T(1620) K L P K K R K R D P G S V G(1634) of human PA200.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proteasome activator subunit 4 , PA200

Cellular Localization

Cytoplasm, cytosol,Nucleus,Nucleus speckle

Background

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. PA200 has been identified as a 200 kDa nuclear protein that activates the 26S proteasome complex. Studies have shown that, in human tissues, PA200 mRNA levels are highest in testis, but is also present in liver, spleen, brain, heart, kidney and lung. Evidence suggests that this protein is involved in DNA repair, possibly by recruiting the proteasome to double-strand DNA breaks.

Database

Research Area

DATA IMAGES

Anti-Proteosome Activator, 200 kDa antibody used in Western Blot (WB). GTX25620

GTX25620 WB Image

WB analysis of 25 ug of mouse testis cell lysates using GTX25620 Proteasome Activator Subunit 4 antibody.
Dilution : 1:2000

Anti-Proteosome Activator, 200 kDa antibody used in Western Blot (WB). GTX25620

GTX25620 WB Image

WB analysis of whole cell extracts (30 ug lysate) of HeLa (Lane 1), Jurkat (Lane 2), and tissue extracts (30 μg lysate) of Mouse testis (Lane 3), Mouse pancreas (Lane 4), and Rat pancreas (Lane 5) using GTX25620 Proteasome Activator Subunit 4 antibody.
Dilution : 1:250

REFERENCE

There are currently no references for Proteosome Activator, 200 kDa antibody (GTX25620). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Proteosome Activator, 200 kDa antibody (GTX25620). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339