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Prothrombin antibody

Anti-Prothrombin antibody used in Flow cytometry (FACS). GTX03694
Anti-Prothrombin antibody used in IHC (Paraffin sections) (IHC-P). GTX03694
Anti-Prothrombin antibody used in Western Blot (WB). GTX03694
Anti-Prothrombin antibody used in IHC (Paraffin sections) (IHC-P). GTX03694

Cat. No. GTX03694

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Human
Package
100 μg ($449)
See all Thrombin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF 0.5-1μg/ml
IHC-P 0.5-1μg/ml
IHC-Fr 0.5-1μg/ml
FACS 1-3μg/1x10⁶ cells
ELISA 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E. coli-derived human Prothrombin recombinant protein (Position: Y67-R124).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coagulation factor II, thrombin , PT , RPRGL2 , THPH1

Background

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Prothrombin antibody used in Flow cytometry (FACS). GTX03694

GTX03694 FACS Image

FACS analysis of human PBMC cells using GTX03694 Prothrombin antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Antibody amount : 1 μg/1x10⁶ cells

Anti-Prothrombin antibody used in IHC (Paraffin sections) (IHC-P). GTX03694

GTX03694 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX03694 Prothrombin antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins

Anti-Prothrombin antibody used in Western Blot (WB). GTX03694

GTX03694 WB Image

WB analysis of human plasma lysate using GTX03694 Prothrombin antibody.
Dilution : 0.5 μg/ml
Loading: 50 μg per lane

Anti-Prothrombin antibody used in IHC (Paraffin sections) (IHC-P). GTX03694

GTX03694 IHC-P Image

IHC-P analysis of human colon cancer tissue using GTX03694 Prothrombin antibody.
Dilution : 1 μg/ml
Antigen retrieval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins

REFERENCE

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REVIEW

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Package List Price ($)
$ 449