APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , human plasma , mouse plasma
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Prothrombin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885226
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
coagulation factor II, thrombin , PT , RPRGL2 , THPH1
Cellular Localization
Secreted , extracellular space
Background
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101199 WB Image
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody (GTX101199) diluted at 1:500.
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GTX101199 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody (GTX101199) diluted at 1:500.
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GTX101199 WB Image
Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Prothrombin antibody (GTX101199) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101199 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
GTX101199 diluted at 1:1000
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REFERENCE
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REVIEW
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