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Prothrombin antibody

Anti-Prothrombin antibody used in Western Blot (WB). GTX101199
Anti-Prothrombin antibody used in Western Blot (WB). GTX101199
Anti-Prothrombin antibody used in Western Blot (WB). GTX101199
Anti-Prothrombin antibody used in Western Blot (WB). GTX101199

Cat No. GTX101199

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all Thrombin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , human plasma , mouse plasma , rat plasma

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Prothrombin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

coagulation factor II, thrombin , PT , RPRGL2 , THPH1

Cellular Localization

Secreted , extracellular space

Background

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Prothrombin antibody used in Western Blot (WB). GTX101199

GTX101199 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody (GTX101199) diluted at 1:500.

Anti-Prothrombin antibody used in Western Blot (WB). GTX101199

GTX101199 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody (GTX101199) diluted at 1:500.

Anti-Prothrombin antibody used in Western Blot (WB). GTX101199

GTX101199 WB Image

Rat plasma (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Prothrombin antibody (GTX101199) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Prothrombin antibody used in Western Blot (WB). GTX101199

GTX101199 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
GTX101199 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169