GeneTex
United States (US)

Protor 1 antibody

GTX31436_2632_WB_20180221_18121410_455.jpg
GTX31436_1053_IHC-P_20180221_18121410_508.jpg
GTX31436_1054_IHC-P_20180221_18121410_172.jpg

Cat No. GTX31436

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

SK-N-SH Cell Lysate(GTX23956)

Specificity/Sensitivity

PRR5 antibody is predicted to not cross-react with other Protor protein family members.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Protor 1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human Protor 1.The immunogen is located within amino acids 310 - 360 of Protor 1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proline Rich 5,Flj20185K,Pp610,Protor-1,Protor1,Prr5

Background

This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES
GTX31436_2632_WB_20180221_18121410_455.jpg

GTX31436 WB Image

WB analysis of SK-N-SH cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31436 Protor 1 antibody.
Working concentration : 1 μg/ml

GTX31436_1053_IHC-P_20180221_18121410_508.jpg

GTX31436 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31436 Protor 1 antibody.
Working concentration : 5 μg/ml

GTX31436_1054_IHC-P_20180221_18121410_172.jpg

GTX31436 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31436 Protor 1 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329