GeneTex
United States (US)

Pumilio 1 antibody [C2C3], C-term

GTX115280_40282_20181004_ICC_IF_R_19011519_134.jpg
GTX115280_WB_18121410_878.jpg
GTX115280_40282_WB_M_Brain_18121410_998.jpg
GTX115280_40282_IHC_18121410_836.jpg
GTX115280_40282_IFA_18121410_949.jpg

Cat No. GTX115280

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Pumilio 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pumilio Rna Binding Family Member 1,Hspum,Pumh,Pumh1,Puml1,Sca47,Pum1

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

DATA IMAGES
GTX115280_40282_20181004_ICC_IF_R_19011519_134.jpg

GTX115280 ICC/IF Image

Pumilio 1 antibody [C2C3], C-term detects Pumilio 1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pumilio 1 stained by Pumilio 1 antibody [C2C3], C-term (GTX115280) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX115280_WB_18121410_878.jpg

GTX115280 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX115280 diluted at 1:1000

GTX115280_40282_WB_M_Brain_18121410_998.jpg

GTX115280 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX115280 diluted at 1:1000

GTX115280_40282_IHC_18121410_836.jpg

GTX115280 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using Pumilio 1(GTX115280) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115280_40282_IFA_18121410_949.jpg

GTX115280 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Pumilio 1(GTX115280) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169