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Pumilio 1 antibody [C2C3], C-term

Anti-Pumilio 1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115280
Anti-Pumilio 1 antibody [C2C3], C-term used in Western Blot (WB). GTX115280
Anti-Pumilio 1 antibody [C2C3], C-term used in Western Blot (WB). GTX115280
Anti-Pumilio 1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX115280
Anti-Pumilio 1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115280

Cat. No. GTX115280

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Pumilio 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Positive Control

Mouse brain , Jurkat

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Pumilio 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624293

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pumilio RNA binding family member 1 , HSPUM , PUMH , PUMH1 , PUML1 , SCA47

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Pumilio 1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115280

GTX115280 ICC/IF Image

Pumilio 1 antibody [C2C3], C-term detects Pumilio 1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pumilio 1 stained by Pumilio 1 antibody [C2C3], C-term (GTX115280) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Pumilio 1 antibody [C2C3], C-term used in Western Blot (WB). GTX115280

GTX115280 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pumilio 1 antibody (GTX115280) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Pumilio 1 antibody [C2C3], C-term used in Western Blot (WB). GTX115280

GTX115280 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX115280 diluted at 1:1000

Anti-Pumilio 1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX115280

GTX115280 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using Pumilio 1(GTX115280) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Pumilio 1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115280

GTX115280 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Pumilio 1(GTX115280) antibody at 1:500 dilution.

REFERENCE

Application Reference

Lee MH et al. J Cell Physiol 2019; RNA-binding protein PUM2 regulates mesenchymal stem cell fate via repression of JAK2 and RUNX2 mRNAs.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169