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Pumilio 2 antibody [C2C3], C-term

Anti-Pumilio 2 antibody [C2C3], C-term used in Western Blot (WB). GTX114172
Anti-Pumilio 2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114172
Anti-Pumilio 2 antibody [C2C3], C-term used in Western Blot (WB). GTX114172

Cat. No. GTX114172

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Pumilio 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

Human ESC , mouse ovary

Predict Reactivity

Rat, Drosophila, Chicken, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Pumilio 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173143

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pumilio RNA binding family member 2 , PUMH2 , PUML2

Cellular Localization

Cytoplasm

Background

Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.

Database

Research Area

DATA IMAGES

Anti-Pumilio 2 antibody [C2C3], C-term used in Western Blot (WB). GTX114172

GTX114172 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pumilio 2 antibody [C2C3], C-term (GTX114172) diluted at 1:500.

Anti-Pumilio 2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114172

GTX114172 ICC/IF Image

Pumilio 2 antibody [C2C3], C-term detects Pumilio 2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Pumilio 2 stained by Pumilio 2 antibody [C2C3], C-term (GTX114172) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Pumilio 2 antibody [C2C3], C-term used in Western Blot (WB). GTX114172

GTX114172 WB Image

Sample (20 ug of whole cell lysate)
A: Human ESC
5% SDS PAGE
GTX114172 diluted at 1:2000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169