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Pumilio 2 antibody, Internal

Anti-Pumilio 2 antibody, Internal used in Western Blot (WB). GTX89543

Cat. No. GTX89543

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-0.5μg/ml
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89543-PEP).

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DQNRDLKQGDDDDSK, from the internal region of the protein sequence according to NP_056132.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pumilio RNA binding family member 2 , PUMH2 , PUML2

Cellular Localization

Cytoplasm

Background

This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Database

Research Area

DATA IMAGES

Anti-Pumilio 2 antibody, Internal used in Western Blot (WB). GTX89543

GTX89543 WB Image

WB analysis of HEK293 overexpressing human Pumilio 2 with DYKDDDDK tag using GTX89543 Pumilio 2 antibody, Internal.
Lane A : primary antibody
Lane B : Mock-transfected HEK293 probed with primary antibody
Lane C : Tag antibody
Dilution : 0.5μg/ml
Loading : 10μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399