GeneTex
United States (US)

Pumilio 2 antibody [N1N2], N-term

GTX107995_39764_20160908_WB_19011507_133.jpg
GTX107995_39764_20141120_IHC_M_19011507_700.jpg
GTX107995_39764_WB_hESC_19011507_615.jpg

Cat No. GTX107995

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

293T , A431 , HeLa , Human ESC
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Pumilio 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pumilio Rna Binding Family Member 2,Pumh2,Puml2,Pum2

Cellular Localization

Cytoplasm

Background

Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.

Database

Research Area

DATA IMAGES
GTX107995_39764_20160908_WB_19011507_133.jpg

GTX107995 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pumilio 2 antibody [N1N2], N-term (GTX107995) diluted at 1:500.

GTX107995_39764_20141120_IHC_M_19011507_700.jpg

GTX107995 IHC-P Image

Pumilio 2 antibody [N1N2], N-term detects Pumilio 2 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Pumilio 2 antibody [N1N2], N-term (GTX107995) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107995_39764_WB_hESC_19011507_615.jpg

GTX107995 WB Image

Sample (20 ug of whole cell lysate)
A: human ESC
5% SDS PAGE
GTX107995 diluted at 1:5000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169