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Purine nucleoside phosphorylase antibody

Anti-Purine nucleoside phosphorylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117364
Anti-Purine nucleoside phosphorylase antibody used in Western Blot (WB). GTX117364

Cat. No. GTX117364

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Purine nucleoside phosphorylase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Purine nucleoside phosphorylase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624658

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

purine nucleoside phosphorylase , NP , PRO1837 , PUNP

Background

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Purine nucleoside phosphorylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117364

GTX117364 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Nucleoside phosphorylase(GTX117364) antibody at 1:500 dilution.

Anti-Purine nucleoside phosphorylase antibody used in Western Blot (WB). GTX117364

GTX117364 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX117364 diluted at 1:1000

REFERENCE

Application Reference

Wang H et al. Am J Cancer Res 2022; 12 (1) : 427-444 Guanosine primes acute myeloid leukemia for differentiation via guanine nucleotide salvage synthesis.
Application : WB
Reactivity : Human
Garcia-Esparcia P et al. Neuropathol Appl Neurobiol 2015; Purine metabolism gene deregulation in Parkinson's disease.
Application : IHC
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169