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Pyruvate Carboxylase antibody, Internal

Anti-Pyruvate Carboxylase antibody, Internal used in Western Blot (WB). GTX88823
Anti-Pyruvate Carboxylase antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88823

Cat No. GTX88823

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.03-0.1μg/ml
IHC-P 5μg/ml
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88823-PEP).

Predict Reactivity

Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KFKEVKKAYVEANQ, from the internal region of the protein sequence according to NP_000911.2; NP_001035806.1; NP_071504.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

pyruvate carboxylase , PCB

Cellular Localization

Mitochondrion matrix

Background

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Carboxylase antibody, Internal used in Western Blot (WB). GTX88823

GTX88823 WB Image

WB analysis of mouse liver lysate using GTX88823 Pyruvate Carboxylase antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Pyruvate Carboxylase antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88823

GTX88823 IHC-P Image

IHC-P analysis of human liver using GTX88823 Pyruvate Carboxylase antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 379