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Pyruvate Carboxylase antibody

Anti-Pyruvate Carboxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX131987
Anti-Pyruvate Carboxylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131987
Anti-Pyruvate Carboxylase antibody used in Western Blot (WB). GTX131987

Cat No. GTX131987

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

Positive Control

293T , A431 , HeLa , mouse liver

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Pyruvate Carboxylase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

pyruvate carboxylase,PCB

Cellular Localization

Mitochondrion matrix

Background

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Pyruvate Carboxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX131987

GTX131987 IHC-P Image

PCB antibody detects PCB protein at mitochondria and cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PCB antibody (GTX131987) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Pyruvate Carboxylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131987

GTX131987 ICC/IF Image

Pyruvate Carboxylase antibody detects Pyruvate Carboxylase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Pyruvate Carboxylase protein stained by Pyruvate Carboxylase antibody (GTX131987) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Pyruvate Carboxylase antibody used in Western Blot (WB). GTX131987

GTX131987 WB Image

PCB antibody detects PCB protein by western blot analysis. Various whole cell extracts (30 μg) and Mouse tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PCB antibody (GTX131987) diluted at a dilution of 1:1000.

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169