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Pyruvate Carboxylase antibody

Anti-Pyruvate Carboxylase antibody used in Western Blot (WB). GTX54577
Anti-Pyruvate Carboxylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54577
Anti-Pyruvate Carboxylase antibody used in Immunoprecipitation (IP). GTX54577

Cat. No. GTX54577

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Pyruvate Carboxylase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

130 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 879-1178 of human PC (NP_001035806.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pyruvate carboxylase , PCB

Cellular Localization

Mitochondrion matrix

Background

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Pyruvate Carboxylase antibody used in Western Blot (WB). GTX54577

GTX54577 WB Image

WB analysis of various sample lysates using GTX54577 Pyruvate Carboxylase antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Pyruvate Carboxylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54577

GTX54577 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54577 Pyruvate Carboxylase antibody.
Blue : DAPI

Anti-Pyruvate Carboxylase antibody used in Immunoprecipitation (IP). GTX54577

GTX54577 IP Image

IP analysis of HeLa cell lysate using GTX54577 Pyruvate Carboxylase antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399